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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF6439 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal CD4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat CD4 in direct ELISAs and Western blots. In direct ELISAs, approximately 3% cross-reacitvity with recombinant feline CD4 is observed, and less than 1% cross-reactivity with recombinant human CD4 and recombinant canine CD4 is observed.
- Reactivity
- Rat
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Rat CD4 by Western Blot. Western blot shows lysates of rat CD4+ T cells and rat spleen tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6439) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of rat CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Sheep Anti-Rat CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6439, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).
