Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- Q10007 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD4 Monoclonal Antibody (S3.5), Qdot™ 655
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- QdotTM Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, QdotTM Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. The isotype control for this antibody is mouse IgG2a, Cat. No. Q10015. Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10007: peak excitation 405 (488) nm/peak emission 655 nm; recommended filter 655/20 nm. Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S3.5
- Vial size
- 100 µL
- Storage
- 4° C
Submitted references T cell phenotypes in women with Chlamydia trachomatis infection and influence of treatment on phenotype distributions.
Distinct peripheral vs mucosal T-cell phenotypes in chlamydia-infected women.
Dual role of novel ingenol derivatives from Euphorbia tirucalli in HIV replication: inhibition of de novo infection and activation of viral LTR.
Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody.
Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.
Heterogeneity of multifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization.
Leukocyte composition of human breast cancer.
Potent CD8+ T-cell immunogenicity in humans of a novel heterosubtypic influenza A vaccine, MVA-NP+M1.
Ogendi BMO, Bakshi RK, Gupta K, Kapil R, Brown LT, Jordan SJ, Sabbaj S, Press CG, Lee JY, Geisler WM
Microbes and infection 2018 Mar;20(3):176-184
Microbes and infection 2018 Mar;20(3):176-184
Distinct peripheral vs mucosal T-cell phenotypes in chlamydia-infected women.
Ogendi BMO, Bakshi RK, Sabbaj S, Brown L, Lee JY, Kapil R, Geisler WM
American journal of reproductive immunology (New York, N.Y. : 1989) 2017 Dec;78(6)
American journal of reproductive immunology (New York, N.Y. : 1989) 2017 Dec;78(6)
Dual role of novel ingenol derivatives from Euphorbia tirucalli in HIV replication: inhibition of de novo infection and activation of viral LTR.
Abreu CM, Price SL, Shirk EN, Cunha RD, Pianowski LF, Clements JE, Tanuri A, Gama L
PloS one 2014;9(5):e97257
PloS one 2014;9(5):e97257
Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody.
Kobie JJ, Treanor JJ, Ritchlin CT
Immunological investigations 2014;43(6):606-15
Immunological investigations 2014;43(6):606-15
Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.
McArthur MA, Sztein MB
Clinical immunology (Orlando, Fla.) 2013 Feb;146(2):140-52
Clinical immunology (Orlando, Fla.) 2013 Feb;146(2):140-52
Heterogeneity of multifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization.
McArthur MA, Sztein MB
PloS one 2012;7(6):e38408
PloS one 2012;7(6):e38408
Leukocyte composition of human breast cancer.
Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, Coussens LM
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):2796-801
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):2796-801
Potent CD8+ T-cell immunogenicity in humans of a novel heterosubtypic influenza A vaccine, MVA-NP+M1.
Berthoud TK, Hamill M, Lillie PJ, Hwenda L, Collins KA, Ewer KJ, Milicic A, Poyntz HC, Lambe T, Fletcher HA, Hill AV, Gilbert SC
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2011 Jan 1;52(1):1-7
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2011 Jan 1;52(1):1-7
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- Invitrogen Antibodies (provider)
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- Experimental details
- Qdot® 655 anti-human CD4 conjugate 655/20 band pass filter