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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB379-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 34930
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Kv1.3 Channels Mark Functionally Competent CD8+ Tumor-Infiltrating Lymphocytes in Head and Neck Cancer.
Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models.
Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity.
Presence of IgG-CD4 complexes in the circulation.
The transmembrane domain of the severe acute respiratory syndrome coronavirus ORF7b protein is necessary and sufficient for its retention in the Golgi complex.
Chimote AA, Hajdu P, Sfyris AM, Gleich BN, Wise-Draper T, Casper KA, Conforti L
Cancer research 2017 Jan 1;77(1):53-61
Cancer research 2017 Jan 1;77(1):53-61
Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models.
Feng Y, Liao S, Wei C, Jia D, Wood K, Liu Q, Wang X, Shi FD, Jin WN
Journal of neuroinflammation 2017 Dec 15;14(1):248
Journal of neuroinflammation 2017 Dec 15;14(1):248
Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity.
Suzuki F, Kubota T, Miyazaki Y, Ishikawa K, Ebisawa M, Hirohata S, Ogura T, Mizusawa H, Imai T, Miyasaka N, Nanki T
Arthritis research & therapy 2012 Mar 6;14(2):R48
Arthritis research & therapy 2012 Mar 6;14(2):R48
Presence of IgG-CD4 complexes in the circulation.
Nezlin R, Bengtsson AA
Immunological investigations 2008;37(2):153-62
Immunological investigations 2008;37(2):153-62
The transmembrane domain of the severe acute respiratory syndrome coronavirus ORF7b protein is necessary and sufficient for its retention in the Golgi complex.
Schaecher SR, Diamond MS, Pekosz A
Journal of virology 2008 Oct;82(19):9477-91
Journal of virology 2008 Oct;82(19):9477-91
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB379) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
