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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BAM554 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD4 Biotinylated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Recognizes mouse CD4 (1-3).
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- GK1.5
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Impaired salivary gland function in NOD mice: association with changes in cytokine profile but not with histopathologic changes in the salivary gland.
Jonsson MV, Delaleu N, Brokstad KA, Berggreen E, Skarstein K
Arthritis and rheumatism 2006 Jul;54(7):2300-5
Arthritis and rheumatism 2006 Jul;54(7):2300-5
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- CD4 in Mouse Splenocytes. CD4 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD4 Biotinylated Monoclonal Antibody (Catalog # BAM554) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
