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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX84714 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84714, RRID:AB_10729852
- Product name
- CD4 antibody [18E3]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 monoclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of live Jurkat cells, using anti-CD4 antibody (GTX84714 at 1:100, Red), compared to IgG1 isotype control (Black).
