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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Flow cytometry [3]
- Other assay [4]
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- Product number
- MHCD0404 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD4 Monoclonal Antibody (S3.5), PE
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients. This antibody may be used, in combination with other indicators, for the diagnosis or prognosis of immunodeficiency diseases, including hypogammaglobulinemia, severe combined immunodeficiency (SCID), and the acquired immunodeficiency disease (AIDS).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- S3.5
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.
Effects of Desflurane and Sevoflurane anesthesia on regulatory T cells in patients undergoing living donor kidney transplantation: a randomized intervention trial.
Secretory High-Mobility Group Box 1 Protein Affects Regulatory T Cell Differentiation in Neuroblastoma Microenvironment In Vitro.
Quality of TCR signaling determined by differential affinities of enhancers for the composite BATF-IRF4 transcription factor complex.
Janus kinase inhibition suppresses PKC-induced cytokine release without affecting HIV-1 latency reversal ex vivo.
TLR and NLRP3 inflammasome-dependent innate immune responses to tumor-derived autophagosomes (DRibbles).
Vpu-Mediated Counteraction of Tetherin Is a Major Determinant of HIV-1 Interferon Resistance.
Non-POU Domain-Containing Octamer-Binding Protein Negatively Regulates HIV-1 Infection in CD4(+) T Cells.
Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.
Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.
Anti-inflammatory/regulatory cytokine microenvironment mediated by IL-4 and IL-10 coordinates the immune response in hemophilia A patients infected chronically with hepatitis C virus.
Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
Immunological/virological peripheral blood biomarkers and distinct patterns of sleeping quality in chronic hepatitis C patients.
The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.
T cell priming enhances IL-4 gene expression by increasing nuclear factor of activated T cells.
Fischer DS, Ansari M, Wagner KI, Jarosch S, Huang Y, Mayr CH, Strunz M, Lang NJ, D'Ippolito E, Hammel M, Mateyka L, Weber S, Wolff LS, Witter K, Fernandez IE, Leuschner G, Milger K, Frankenberger M, Nowak L, Heinig-Menhard K, Koch I, Stoleriu MG, Hilgendorff A, Behr J, Pichlmair A, Schubert B, Theis FJ, Busch DH, Schiller HB, Schober K
Nature communications 2021 Jul 26;12(1):4515
Nature communications 2021 Jul 26;12(1):4515
Effects of Desflurane and Sevoflurane anesthesia on regulatory T cells in patients undergoing living donor kidney transplantation: a randomized intervention trial.
Chutipongtanate A, Prukviwat S, Pongsakul N, Srisala S, Kamanee N, Arpornsujaritkun N, Gesprasert G, Apiwattanakul N, Hongeng S, Ittichaikulthol W, Sumethkul V, Chutipongtanate S
BMC anesthesiology 2020 Aug 27;20(1):215
BMC anesthesiology 2020 Aug 27;20(1):215
Secretory High-Mobility Group Box 1 Protein Affects Regulatory T Cell Differentiation in Neuroblastoma Microenvironment In Vitro.
Vanichapol T, Chiangjong W, Panachan J, Anurathapan U, Chutipongtanate S, Hongeng S
Journal of oncology 2018;2018:7946021
Journal of oncology 2018;2018:7946021
Quality of TCR signaling determined by differential affinities of enhancers for the composite BATF-IRF4 transcription factor complex.
Iwata A, Durai V, Tussiwand R, Briseño CG, Wu X, Grajales-Reyes GE, Egawa T, Murphy TL, Murphy KM
Nature immunology 2017 May;18(5):563-572
Nature immunology 2017 May;18(5):563-572
Janus kinase inhibition suppresses PKC-induced cytokine release without affecting HIV-1 latency reversal ex vivo.
Spivak AM, Larragoite ET, Coletti ML, Macedo AB, Martins LJ, Bosque A, Planelles V
Retrovirology 2016 Dec 20;13(1):88
Retrovirology 2016 Dec 20;13(1):88
TLR and NLRP3 inflammasome-dependent innate immune responses to tumor-derived autophagosomes (DRibbles).
Xing Y, Cao R, Hu HM
Cell death & disease 2016 Aug 4;7(8):e2322
Cell death & disease 2016 Aug 4;7(8):e2322
Vpu-Mediated Counteraction of Tetherin Is a Major Determinant of HIV-1 Interferon Resistance.
Kmiec D, Iyer SS, Stürzel CM, Sauter D, Hahn BH, Kirchhoff F
mBio 2016 Aug 16;7(4)
mBio 2016 Aug 16;7(4)
Non-POU Domain-Containing Octamer-Binding Protein Negatively Regulates HIV-1 Infection in CD4(+) T Cells.
St Gelais C, Roger J, Wu L
AIDS research and human retroviruses 2015 Aug;31(8):806-16
AIDS research and human retroviruses 2015 Aug;31(8):806-16
Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.
de Carvalho JV, de Castro RO, da Silva EZ, Silveira PP, da Silva-Januário ME, Arruda E, Jamur MC, Oliver C, Aguiar RS, daSilva LL
PloS one 2014;9(11):e113691
PloS one 2014;9(11):e113691
Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.
Haldar M, Kohyama M, So AY, Kc W, Wu X, Briseño CG, Satpathy AT, Kretzer NM, Arase H, Rajasekaran NS, Wang L, Egawa T, Igarashi K, Baltimore D, Murphy TL, Murphy KM
Cell 2014 Mar 13;156(6):1223-1234
Cell 2014 Mar 13;156(6):1223-1234
Anti-inflammatory/regulatory cytokine microenvironment mediated by IL-4 and IL-10 coordinates the immune response in hemophilia A patients infected chronically with hepatitis C virus.
Pimentel JP, Chaves DG, Araújo AR, de Araújo EM, da Silva Fraporti L, Neves WL, Tarragô AM, Torres KL, Gentz SH, Teixeira-Carvalho A, Martins-Filho OA, Malheiro A
Journal of medical virology 2013 Jun;85(6):1009-18
Journal of medical virology 2013 Jun;85(6):1009-18
Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE
PloS one 2011;6(8):e22848
PloS one 2011;6(8):e22848
Immunological/virological peripheral blood biomarkers and distinct patterns of sleeping quality in chronic hepatitis C patients.
de Almeida CM, de Lima TA, Castro DB, Torres KL, da Silva Braga W, Peruhype-Magalhães V, Teixeira-Carvalho A, Martins-Filho OA, Malheiro A
Scandinavian journal of immunology 2011 May;73(5):486-95
Scandinavian journal of immunology 2011 May;73(5):486-95
The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.
Elsner L, Flügge PF, Lozano J, Muppala V, Eiz-Vesper B, Demiroglu SY, Malzahn D, Herrmann T, Brunner E, Bickeböller H, Multhoff G, Walter L, Dressel R
Journal of cellular and molecular medicine 2010 Apr;14(4):992-1002
Journal of cellular and molecular medicine 2010 Apr;14(4):992-1002
T cell priming enhances IL-4 gene expression by increasing nuclear factor of activated T cells.
Cron RQ, Bort SJ, Wang Y, Brunvand MW, Lewis DB
Journal of immunology (Baltimore, Md. : 1950) 1999 Jan 15;162(2):860-70
Journal of immunology (Baltimore, Md. : 1950) 1999 Jan 15;162(2):860-70
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry histogram showing human mononuclear cells stained with anti-CD4 complexed with Alexa Fluor® 700 Zenon® labeling reagent. An antibody complex was made by using the Zenon® Alexa Fluor® 700 Mouse IgG1 Labeling Kit (Cat. no. Z25011) and mouse anti human CD4. Human mononuclear cells were stained with the antibody complex and analyzed by flow cytometry using a 633 nm laser.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were stained using R-PE of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry histogram showing human mononuclear cells stained with anti-CD4 complexed with Alexa Fluor® 700 Zenon® labeling reagent. An antibody complex was made by using the Zenon® Alexa Fluor® 700 Mouse IgG1 Labeling Kit (Product # Z-25011) and mouse anti human CD4. Human mononuclear cells were stained with the antibody complex and analyzed by flow cytometry using a 633 nm laser.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Endocytosis was necessary in the process of DRibbles-inflammasome response. ( a ) CypHer5E-labeled DRibbles and lysotraker Green-labeled lysosome were collocated in THP-1 cells after 6 h incubation, and the nucleus were labeled with DAPI. ( b ) The endocytosis frequency of DRibbles by THP-1 cells was measured by FACS. DCs were pre-treated with ExoA (1 mu M), Cytochalasin-D (Cyto-D, 1 mu g/ml), and NH 4 Cl (20 mu M) for 30 min, then incubated with CypHer5E stained DRibbles (100 mu g/ml) overnight. ( c ) Primed THP-1 cells were pre-treated with cytochalasin-D, ExoA, and NH 4 Cl for 1 h, then stimulated with ATP/LPS (5 mM)/(10 ng/ml) and DRibbles (100 mu g/ml) overnight. The supernatant IL-1 beta was detected by ELISA. ( d ) The frequency of IFN- gamma + CD8 + , IFN- gamma + CD4 + T cells induced by pp65 DRibbles at indicated doses were measured by ICS, and ( e ) pp65 protein (10 mu g/ml) and pp65 DRibbles (10 mu g/ml). Results are shown as the means+S.D. and representative of two or more independent experiments. PBMC is from 3 donors
- Conjugate
- Yellow dye
