MHCD0404-4

antibody from Invitrogen Antibodies

Targeting: CD4

Antibody data

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Validation data

Product number: MHCD0404-4 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD4 Monoclonal Antibody (S3.5), PE
Antibody type: Monoclonal
Antigen: Other
Description: R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
Conjugate: Yellow dye
Antibody clone number: S3.5

CD4 protein structure - MHCD0404-4 shown in red.

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Endocytosis was necessary in the process of DRibbles-inflammasome response. ( a ) CypHer5E-labeled DRibbles and lysotraker Green-labeled lysosome were collocated in THP-1 cells after 6 h incubation, and the nucleus were labeled with DAPI. ( b ) The endocytosis frequency of DRibbles by THP-1 cells was measured by FACS. DCs were pre-treated with ExoA (1 mu M), Cytochalasin-D (Cyto-D, 1 mu g/ml), and NH 4 Cl (20 mu M) for 30 min, then incubated with CypHer5E stained DRibbles (100 mu g/ml) overnight. ( c ) Primed THP-1 cells were pre-treated with cytochalasin-D, ExoA, and NH 4 Cl for 1 h, then stimulated with ATP/LPS (5 mM)/(10 ng/ml) and DRibbles (100 mu g/ml) overnight. The supernatant IL-1 beta was detected by ELISA. ( d ) The frequency of IFN- gamma + CD8 + , IFN- gamma + CD4 + T cells induced by pp65 DRibbles at indicated doses were measured by ICS, and ( e ) pp65 protein (10 mu g/ml) and pp65 DRibbles (10 mu g/ml). Results are shown as the means+S.D. and representative of two or more independent experiments. PBMC is from 3 donors
Conjugate: Yellow dye