Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MHCD0430 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD4 Monoclonal Antibody (S3.5), Pacific Orange™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S3.5
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.
Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype.
Mycobacterium tuberculosis-specific and MHC class I-restricted CD8+ T-cells exhibit a stem cell precursor-like phenotype in patients with active pulmonary tuberculosis.
Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults.
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.
Detection of JC virus-specific immune responses in a novel humanized mouse model.
Low levels of peripheral CD161++CD8+ mucosal associated invariant T (MAIT) cells are found in HIV and HIV/TB co-infection.
Vitamin B6 and the immunity in kidney transplant recipients.
Expression of integrin α2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2Rγ(c) null mice.
Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.
Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques.
HIV-induced changes in T cell signaling pathways.
HIV-induced changes in T cell signaling pathways.
Increased frequency of EBV-specific effector memory CD8+ T cells correlates with higher viral load in rheumatoid arthritis.
Increased frequency of EBV-specific effector memory CD8+ T cells correlates with higher viral load in rheumatoid arthritis.
Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey).
High expression of Fas ligand by synovial fluid-derived gamma delta T cells in Lyme arthritis.
High expression of Fas ligand by synovial fluid-derived gamma delta T cells in Lyme arthritis.
CD4+CD25+ regulatory T cells do not significantly contribute to direct pathway hyporesponsiveness in stable renal transplant patients.
CD4+CD25+ regulatory T cells do not significantly contribute to direct pathway hyporesponsiveness in stable renal transplant patients.
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.
Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.
Unique CD8+ T cell-rich lymphoid aggregates in human uterine endometrium.
Grieco AJ, Billett HH, Green NS, Driscoll MC, Bouhassira EE
PloS one 2015;10(6):e0129431
PloS one 2015;10(6):e0129431
Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype.
Riou C, Tanko RF, Soares AP, Masson L, Werner L, Garrett NJ, Samsunder N, Karim QA, Karim SSA, Burgers WA
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2273-2281
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2273-2281
Mycobacterium tuberculosis-specific and MHC class I-restricted CD8+ T-cells exhibit a stem cell precursor-like phenotype in patients with active pulmonary tuberculosis.
Axelsson-Robertson R, Ju JH, Kim HY, Zumla A, Maeurer M
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 2015 Mar;32:13-22
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 2015 Mar;32:13-22
Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults.
Boer MC, Prins C, van Meijgaarden KE, van Dissel JT, Ottenhoff TH, Joosten SA
Clinical and vaccine immunology : CVI 2015 Jul;22(7):778-88
Clinical and vaccine immunology : CVI 2015 Jul;22(7):778-88
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
Pegram HJ, Purdon TJ, van Leeuwen DG, Curran KJ, Giralt SA, Barker JN, Brentjens RJ
Leukemia 2015 Feb;29(2):415-22
Leukemia 2015 Feb;29(2):415-22
Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.
de Carvalho JV, de Castro RO, da Silva EZ, Silveira PP, da Silva-Januário ME, Arruda E, Jamur MC, Oliver C, Aguiar RS, daSilva LL
PloS one 2014;9(11):e113691
PloS one 2014;9(11):e113691
Detection of JC virus-specific immune responses in a novel humanized mouse model.
Tan CS, Broge TA Jr, Seung E, Vrbanac V, Viscidi R, Gordon J, Tager AM, Koralnik IJ
PloS one 2013;8(5):e64313
PloS one 2013;8(5):e64313
Low levels of peripheral CD161++CD8+ mucosal associated invariant T (MAIT) cells are found in HIV and HIV/TB co-infection.
Wong EB, Akilimali NA, Govender P, Sullivan ZA, Cosgrove C, Pillay M, Lewinsohn DM, Bishai WR, Walker BD, Ndung'u T, Klenerman P, Kasprowicz VO
PloS one 2013;8(12):e83474
PloS one 2013;8(12):e83474
Vitamin B6 and the immunity in kidney transplant recipients.
Jankowska M, Marszałł M, Dębska-Ślizień A, Carrero JJ, Lindholm B, Czarnowski W, Rutkowski B, Trzonkowski P
Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation 2013 Jan;23(1):57-64
Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation 2013 Jan;23(1):57-64
Expression of integrin α2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2Rγ(c) null mice.
Wong WM, Sigvardsson M, Åstrand-Grundström I, Hogge D, Larsson J, Qian H, Ekblom M
Stem cells (Dayton, Ohio) 2013 Feb;31(2):360-71
Stem cells (Dayton, Ohio) 2013 Feb;31(2):360-71
Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.
McArthur MA, Sztein MB
Clinical immunology (Orlando, Fla.) 2013 Feb;146(2):140-52
Clinical immunology (Orlando, Fla.) 2013 Feb;146(2):140-52
Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques.
Cornwell WD, Lewis MG, Fan X, Rappaport J, Rogers TJ
Journal of neuroimmunology 2013 Dec 15;265(1-2):43-50
Journal of neuroimmunology 2013 Dec 15;265(1-2):43-50
HIV-induced changes in T cell signaling pathways.
Schweneker M, Favre D, Martin JN, Deeks SG, McCune JM
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6490-500
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6490-500
HIV-induced changes in T cell signaling pathways.
Schweneker M, Favre D, Martin JN, Deeks SG, McCune JM
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6490-500
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6490-500
Increased frequency of EBV-specific effector memory CD8+ T cells correlates with higher viral load in rheumatoid arthritis.
Lünemann JD, Frey O, Eidner T, Baier M, Roberts S, Sashihara J, Volkmer R, Cohen JI, Hein G, Kamradt T, Münz C
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):991-1000
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):991-1000
Increased frequency of EBV-specific effector memory CD8+ T cells correlates with higher viral load in rheumatoid arthritis.
Lünemann JD, Frey O, Eidner T, Baier M, Roberts S, Sashihara J, Volkmer R, Cohen JI, Hein G, Kamradt T, Münz C
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):991-1000
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):991-1000
Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey).
Contamin H, Loizon S, Bourreau E, Michel JC, Garraud O, Mercereau-Puijalon O, Behr C
Journal of immunological methods 2005 Feb;297(1-2):61-71
Journal of immunological methods 2005 Feb;297(1-2):61-71
High expression of Fas ligand by synovial fluid-derived gamma delta T cells in Lyme arthritis.
Roessner K, Wolfe J, Shi C, Sigal LH, Huber S, Budd RC
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2702-10
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2702-10
High expression of Fas ligand by synovial fluid-derived gamma delta T cells in Lyme arthritis.
Roessner K, Wolfe J, Shi C, Sigal LH, Huber S, Budd RC
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2702-10
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2702-10
CD4+CD25+ regulatory T cells do not significantly contribute to direct pathway hyporesponsiveness in stable renal transplant patients.
Game DS, Hernandez-Fuentes MP, Chaudhry AN, Lechler RI
Journal of the American Society of Nephrology : JASN 2003 Jun;14(6):1652-61
Journal of the American Society of Nephrology : JASN 2003 Jun;14(6):1652-61
CD4+CD25+ regulatory T cells do not significantly contribute to direct pathway hyporesponsiveness in stable renal transplant patients.
Game DS, Hernandez-Fuentes MP, Chaudhry AN, Lechler RI
Journal of the American Society of Nephrology : JASN 2003 Jun;14(6):1652-61
Journal of the American Society of Nephrology : JASN 2003 Jun;14(6):1652-61
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
Telford WG, Moss MW, Morseman JP, Allnutt FC
Cytometry 2001 May 1;44(1):16-23
Cytometry 2001 May 1;44(1):16-23
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
Telford WG, Moss MW, Morseman JP, Allnutt FC
Cytometry 2001 May 1;44(1):16-23
Cytometry 2001 May 1;44(1):16-23
Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.
Le Cleach L, Delaire S, Boumsell L, Bagot M, Bourgault-Villada I, Bensussan A, Roujeau JC
Clinical and experimental immunology 2000 Jan;119(1):225-30
Clinical and experimental immunology 2000 Jan;119(1):225-30
Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.
Le Cleach L, Delaire S, Boumsell L, Bagot M, Bourgault-Villada I, Bensussan A, Roujeau JC
Clinical and experimental immunology 2000 Jan;119(1):225-30
Clinical and experimental immunology 2000 Jan;119(1):225-30
Unique CD8+ T cell-rich lymphoid aggregates in human uterine endometrium.
Yeaman GR, Guyre PM, Fanger MW, Collins JE, White HD, Rathbun W, Orndorff KA, Gonzalez J, Stern JE, Wira CR
Journal of leukocyte biology 1997 Apr;61(4):427-35
Journal of leukocyte biology 1997 Apr;61(4):427-35
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse Anti-Humana CD4 Pacific Orange-H Histogram.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis using a CD4 monoclonal antibody (Product # MHCD0430).
