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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB3791V-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD4 Alexa Fluor® 405-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD4 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Blue dye
- Antigen sequence
P01730- Isotype
- IgG
- Antibody clone number
- 11830
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references VSIG-3 as a ligand of VISTA inhibits human T-cell function.
Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
Immunology 2019 Jan;156(1):74-85
Immunology 2019 Jan;156(1):74-85
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of CD4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB3791V) or (B) Mouse IgG2A Alexa Fluor 405 Isotype Control (Catalog # IC003V). View our protocol for Staining Membrane-associated Proteins.
