PAB3534

antibody from Abnova Corporation

Targeting: CD4

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3534

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3534, RRID:AB_1572334

Product name

CD4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of CD4.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Structural basis for the interaction between focal adhesion kinase and CD4.

Garron ML, Arthos J, Guichou JF, McNally J, Cicala C, Arold ST
Journal of molecular biology 2008 Feb 1;375(5):1320-8

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Sequence variation occurs in CD4 epitopes during early HIV infection.

Rychert J, Saindon S, Placek S, Daskalakis D, Rosenberg E
Journal of acquired immune deficiency syndromes (1999) 2007 Nov 1;46(3):261-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of CD4 polyclonal antibody (Cat # PAB3534) in HeLa cell line lysates (35 ug/lane). CD4 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

(RIGHT) Western blot analysis of CD4 (arrow) using rabbit CD4 polyclonal antibody (Cat # PAB3534). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies).

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Flow cytometric analysis of CEM cells using CD4 polyclonal antibody (Cat # PAB3534)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Validation comment

Flow Cytometry