22-0425-73

antibody from Invitrogen Antibodies

Targeting: CD4

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

22-0425-73

Provider

Invitrogen Antibodies

Product name

CD4/CD25 Antibody Cocktail, APC, FITC, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to identify CD4+CD25+ human peripheral blood cells. The RPA-T4 monoclonal antibody (mouse IgG1, kappa) reacts with human CD4, a 59 kDa cell surface receptor that is expressed by a majority of thymocytes, a subpopulation of mature T cells (T helper cells) and at low levels on monocytes. The BC96 monoclonal antibody (mouse IgG1, kappa) reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). This cocktail of RPA-T4 and BC96 can be used for the flow cytometric detection of human regulatory T cells, which also express Foxp3.

Antibody clone number

RPA-T4, BC96

Concentration

20 µL/Test

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue and CD4/CD25 APC/FITC cocktail. As expected based on known relative expression patterns, CD4 clone RPA-T4 and CD25 clone BC96 stains a subset of lymphocytes, respectively.