Antibody data

Product number
22-0425-73 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD4/CD25 Antibody Cocktail, APC, FITC, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to identify CD4+CD25+ human peripheral blood cells. The RPA-T4 monoclonal antibody (mouse IgG1, kappa) reacts with human CD4, a 59 kDa cell surface receptor that is expressed by a majority of thymocytes, a subpopulation of mature T cells (T helper cells) and at low levels on monocytes. The BC96 monoclonal antibody (mouse IgG1, kappa) reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). This cocktail of RPA-T4 and BC96 can be used for the flow cytometric detection of human regulatory T cells, which also express Foxp3. Applications Reported: This product has been reported for use in flow cytometric analysis. Applications Tested: This cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test (containing 1 µg of Anti-Human CD4 (RPA-T4) FITC and 0.125 µg of Anti-Human CD25 (BC96) APC). A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
RPA-T4, BC96
Vial size
100 Tests
Concentration
20 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD4 protein structure - 22-0425-73 shown in red.