Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations [0]
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- Product number
- 53-0048-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD4 Monoclonal Antibody (OKT4 (OKT-4)), Alexa Fluor 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis. Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- OKT4 (OKT-4)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism.
Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.
IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.
Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.
PD-L1 expression and prognostic impact in glioblastoma.
Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.
The HIV-1 gp120 CD4-bound conformation is preferentially targeted by antibody-dependent cellular cytotoxicity-mediating antibodies in sera from HIV-1-infected individuals.
An increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma.
Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Proportions of CD4+ memory T cells are altered in individuals chronically infected with Schistosoma haematobium.
Expansion of CD4+CD25+FOXP3+ regulatory T cells in infants of mothers with type 1 diabetes.
HIV envelope gp120 activates LFA-1 on CD4 T-lymphocytes and increases cell susceptibility to LFA-1-targeting leukotoxin (LtxA).
Regulatory and activated T cells in human Schistosoma haematobium infections.
CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.
Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1.
Arbore G, West EE, Rahman J, Le Friec G, Niyonzima N, Pirooznia M, Tunc I, Pavlidis P, Powell N, Li Y, Liu P, Servais A, Couzi L, Fremeaux-Bacchi V, Placais L, Ferraro A, Walsh PR, Kavanagh D, Afzali B, Lavender P, Lachmann HJ, Kemper C
Nature communications 2018 Oct 10;9(1):4186
Nature communications 2018 Oct 10;9(1):4186
Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.
Chen X, Wang Y, Wang J, Wen J, Jia X, Wang X, Zhang H
Oncology letters 2018 Jul;16(1):253-261
Oncology letters 2018 Jul;16(1):253-261
IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.
Hsieh WC, Hsu TS, Chang YJ, Lai MZ
Nature communications 2018 Jan 31;9(1):463
Nature communications 2018 Jan 31;9(1):463
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Li Y, Brauer PM, Singh J, Xhiku S, Yoganathan K, Zúñiga-Pflücker JC, Anderson MK
Stem cell reports 2017 Sep 12;9(3):779-795
Stem cell reports 2017 Sep 12;9(3):779-795
Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.
Tischner D, Grimm M, Kaur H, Staudenraus D, Carvalho J, Looso M, Günther S, Wanke F, Moos S, Siller N, Breuer J, Schwab N, Zipp F, Waisman A, Kurschus FC, Offermanns S, Wettschureck N
JCI insight 2017 Aug 3;2(15)
JCI insight 2017 Aug 3;2(15)
Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.
Quintremil S, Alberti C, Rivera M, Medina F, Puente J, Cartier L, Ramírez E, Tanaka Y, Valenzuela MA
AIDS research and human retroviruses 2016 Jan;32(1):68-79
AIDS research and human retroviruses 2016 Jan;32(1):68-79
PD-L1 expression and prognostic impact in glioblastoma.
Nduom EK, Wei J, Yaghi NK, Huang N, Kong LY, Gabrusiewicz K, Ling X, Zhou S, Ivan C, Chen JQ, Burks JK, Fuller GN, Calin GA, Conrad CA, Creasy C, Ritthipichai K, Radvanyi L, Heimberger AB
Neuro-oncology 2016 Feb;18(2):195-205
Neuro-oncology 2016 Feb;18(2):195-205
Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.
Muller L, Mitsuhashi M, Simms P, Gooding WE, Whiteside TL
Scientific reports 2016 Feb 4;6:20254
Scientific reports 2016 Feb 4;6:20254
The HIV-1 gp120 CD4-bound conformation is preferentially targeted by antibody-dependent cellular cytotoxicity-mediating antibodies in sera from HIV-1-infected individuals.
Veillette M, Coutu M, Richard J, Batraville LA, Dagher O, Bernard N, Tremblay C, Kaufmann DE, Roger M, Finzi A
Journal of virology 2015 Jan;89(1):545-51
Journal of virology 2015 Jan;89(1):545-51
An increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma.
Tang Y, Xu X, Guo S, Zhang C, Tang Y, Tian Y, Ni B, Lu B, Wang H
PloS one 2014;9(3):e91551
PloS one 2014;9(3):e91551
Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.
Wertheimer AM, Bennett MS, Park B, Uhrlaub JL, Martinez C, Pulko V, Currier NL, Nikolich-Žugich D, Kaye J, Nikolich-Žugich J
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2143-55
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2143-55
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Palin AC, Ramachandran V, Acharya S, Lewis DB
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
Proportions of CD4+ memory T cells are altered in individuals chronically infected with Schistosoma haematobium.
Nausch N, Bourke CD, Appleby LJ, Rujeni N, Lantz O, Trottein F, Midzi N, Mduluza T, Mutapi F
Scientific reports 2012;2:472
Scientific reports 2012;2:472
Expansion of CD4+CD25+FOXP3+ regulatory T cells in infants of mothers with type 1 diabetes.
Luopajärvi K, Nieminen JK, Ilonen J, Akerblom HK, Knip M, Vaarala O
Pediatric diabetes 2012 Aug;13(5):400-7
Pediatric diabetes 2012 Aug;13(5):400-7
HIV envelope gp120 activates LFA-1 on CD4 T-lymphocytes and increases cell susceptibility to LFA-1-targeting leukotoxin (LtxA).
Hioe CE, Tuen M, Vasiliver-Shamis G, Alvarez Y, Prins KC, Banerjee S, Nádas A, Cho MW, Dustin ML, Kachlany SC
PloS one 2011;6(8):e23202
PloS one 2011;6(8):e23202
Regulatory and activated T cells in human Schistosoma haematobium infections.
Nausch N, Midzi N, Mduluza T, Maizels RM, Mutapi F
PloS one 2011 Feb 10;6(2):e16860
PloS one 2011 Feb 10;6(2):e16860
CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.
Achour L, Scott MG, Shirvani H, Thuret A, Bismuth G, Labbé-Jullié C, Marullo S
Blood 2009 Feb 26;113(9):1938-47
Blood 2009 Feb 26;113(9):1938-47
Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1.
Bour S, Boulerice F, Wainberg MA
Journal of virology 1991 Dec;65(12):6387-96
Journal of virology 1991 Dec;65(12):6387-96
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