Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 56-0048-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD4 Monoclonal Antibody (OKT4 (OKT-4)), Alexa Fluor 700, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis. Applications Tested: This OKT4 (OKT-4) antibody is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Near infrared dye
- Isotype
- IgG
- Antibody clone number
- OKT4 (OKT-4)
- Vial size
- 25 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Complement receptor CD46 co-stimulates optimal human CD8(+) T cell effector function via fatty acid metabolism.
Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.
IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.
The influence of chemotherapy on adenosine-producing B cells in patients with head and neck squamous cell carcinoma.
The chimeric TAC receptor co-opts the T cell receptor yielding robust anti-tumor activity without toxicity.
Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.
Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?
Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.
PD-L1 expression and prognostic impact in glioblastoma.
Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.
Impaired survival of regulatory T cells in pulmonary sarcoidosis.
The HIV-1 gp120 CD4-bound conformation is preferentially targeted by antibody-dependent cellular cytotoxicity-mediating antibodies in sera from HIV-1-infected individuals.
An increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma.
Comparative immune phenotypic analysis of cutaneous Squamous Cell Carcinoma and Intraepidermal Carcinoma in immune-competent individuals: proportional representation of CD8+ T-cells but not FoxP3+ Regulatory T-cells is associated with disease stage.
Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Capsid-specific T-cell responses to natural infections with adeno-associated viruses in humans differ from those of nonhuman primates.
CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.
Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1.
Arbore G, West EE, Rahman J, Le Friec G, Niyonzima N, Pirooznia M, Tunc I, Pavlidis P, Powell N, Li Y, Liu P, Servais A, Couzi L, Fremeaux-Bacchi V, Placais L, Ferraro A, Walsh PR, Kavanagh D, Afzali B, Lavender P, Lachmann HJ, Kemper C
Nature communications 2018 Oct 10;9(1):4186
Nature communications 2018 Oct 10;9(1):4186
Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.
Chen X, Wang Y, Wang J, Wen J, Jia X, Wang X, Zhang H
Oncology letters 2018 Jul;16(1):253-261
Oncology letters 2018 Jul;16(1):253-261
IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.
Hsieh WC, Hsu TS, Chang YJ, Lai MZ
Nature communications 2018 Jan 31;9(1):463
Nature communications 2018 Jan 31;9(1):463
The influence of chemotherapy on adenosine-producing B cells in patients with head and neck squamous cell carcinoma.
Ziebart A, Huber U, Jeske S, Laban S, Doescher J, Hoffmann TK, Brunner C, Jackson EK, Schuler PJ
Oncotarget 2018 Jan 19;9(5):5834-5847
Oncotarget 2018 Jan 19;9(5):5834-5847
The chimeric TAC receptor co-opts the T cell receptor yielding robust anti-tumor activity without toxicity.
Helsen CW, Hammill JA, Lau VWC, Mwawasi KA, Afsahi A, Bezverbnaya K, Newhook L, Hayes DL, Aarts C, Bojovic B, Denisova GF, Kwiecien JM, Brain I, Derocher H, Milne K, Nelson BH, Bramson JL
Nature communications 2018 Aug 3;9(1):3049
Nature communications 2018 Aug 3;9(1):3049
Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.
Arce Vargas F, Furness AJS, Litchfield K, Joshi K, Rosenthal R, Ghorani E, Solomon I, Lesko MH, Ruef N, Roddie C, Henry JY, Spain L, Ben Aissa A, Georgiou A, Wong YNS, Smith M, Strauss D, Hayes A, Nicol D, O'Brien T, Mårtensson L, Ljungars A, Teige I, Frendéus B, TRACERx Melanoma., TRACERx Renal., TRACERx Lung consortia., Pule M, Marafioti T, Gore M, Larkin J, Turajlic S, Swanton C, Peggs KS, Quezada SA
Cancer cell 2018 Apr 9;33(4):649-663.e4
Cancer cell 2018 Apr 9;33(4):649-663.e4
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Li Y, Brauer PM, Singh J, Xhiku S, Yoganathan K, Zúñiga-Pflücker JC, Anderson MK
Stem cell reports 2017 Sep 12;9(3):779-795
Stem cell reports 2017 Sep 12;9(3):779-795
Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.
Tischner D, Grimm M, Kaur H, Staudenraus D, Carvalho J, Looso M, Günther S, Wanke F, Moos S, Siller N, Breuer J, Schwab N, Zipp F, Waisman A, Kurschus FC, Offermanns S, Wettschureck N
JCI insight 2017 Aug 3;2(15)
JCI insight 2017 Aug 3;2(15)
Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?
Stikvoort A, Sundin M, Uzunel M, Gertow J, Sundberg B, Schaffer M, Mattsson J, Uhlin M
PloS one 2016;11(5):e0154737
PloS one 2016;11(5):e0154737
Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.
Quintremil S, Alberti C, Rivera M, Medina F, Puente J, Cartier L, Ramírez E, Tanaka Y, Valenzuela MA
AIDS research and human retroviruses 2016 Jan;32(1):68-79
AIDS research and human retroviruses 2016 Jan;32(1):68-79
PD-L1 expression and prognostic impact in glioblastoma.
Nduom EK, Wei J, Yaghi NK, Huang N, Kong LY, Gabrusiewicz K, Ling X, Zhou S, Ivan C, Chen JQ, Burks JK, Fuller GN, Calin GA, Conrad CA, Creasy C, Ritthipichai K, Radvanyi L, Heimberger AB
Neuro-oncology 2016 Feb;18(2):195-205
Neuro-oncology 2016 Feb;18(2):195-205
Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.
Muller L, Mitsuhashi M, Simms P, Gooding WE, Whiteside TL
Scientific reports 2016 Feb 4;6:20254
Scientific reports 2016 Feb 4;6:20254
Impaired survival of regulatory T cells in pulmonary sarcoidosis.
Broos CE, van Nimwegen M, Kleinjan A, ten Berge B, Muskens F, in 't Veen JC, Annema JT, Lambrecht BN, Hoogsteden HC, Hendriks RW, Kool M, van den Blink B
Respiratory research 2015 Sep 16;16:108
Respiratory research 2015 Sep 16;16:108
The HIV-1 gp120 CD4-bound conformation is preferentially targeted by antibody-dependent cellular cytotoxicity-mediating antibodies in sera from HIV-1-infected individuals.
Veillette M, Coutu M, Richard J, Batraville LA, Dagher O, Bernard N, Tremblay C, Kaufmann DE, Roger M, Finzi A
Journal of virology 2015 Jan;89(1):545-51
Journal of virology 2015 Jan;89(1):545-51
An increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma.
Tang Y, Xu X, Guo S, Zhang C, Tang Y, Tian Y, Ni B, Lu B, Wang H
PloS one 2014;9(3):e91551
PloS one 2014;9(3):e91551
Comparative immune phenotypic analysis of cutaneous Squamous Cell Carcinoma and Intraepidermal Carcinoma in immune-competent individuals: proportional representation of CD8+ T-cells but not FoxP3+ Regulatory T-cells is associated with disease stage.
Freeman A, Bridge JA, Maruthayanar P, Overgaard NH, Jung JW, Simpson F, Prow TW, Soyer HP, Frazer IH, Freeman M, Wells JW
PloS one 2014;9(10):e110928
PloS one 2014;9(10):e110928
Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.
Wertheimer AM, Bennett MS, Park B, Uhrlaub JL, Martinez C, Pulko V, Currier NL, Nikolich-Žugich D, Kaye J, Nikolich-Žugich J
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2143-55
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2143-55
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Palin AC, Ramachandran V, Acharya S, Lewis DB
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
Capsid-specific T-cell responses to natural infections with adeno-associated viruses in humans differ from those of nonhuman primates.
Li H, Lasaro MO, Jia B, Lin SW, Haut LH, High KA, Ertl HC
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Nov;19(11):2021-30
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Nov;19(11):2021-30
CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.
Achour L, Scott MG, Shirvani H, Thuret A, Bismuth G, Labbé-Jullié C, Marullo S
Blood 2009 Feb 26;113(9):1938-47
Blood 2009 Feb 26;113(9):1938-47
Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1.
Bour S, Boulerice F, Wainberg MA
Journal of virology 1991 Dec;65(12):6387-96
Journal of virology 1991 Dec;65(12):6387-96
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and staining buffer (autofluorescence) (left) or Anti-Human CD4 Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Near infrared dye