58-0047-42

antibody from Invitrogen Antibodies

Targeting: CD4

Provider product page for 58-0047-42

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [5]
Comments [0]
Validations
Flow cytometry [1]
Other assay [4]

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Product number: 58-0047-42 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD4 Monoclonal Antibody (SK3 (SK-3)), Alexa Fluor™ 532, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes. Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis. Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser
Reactivity: Human
Host: Mouse
Conjugate: Yellow dye
Isotype: IgG
Antibody clone number: SK3 (SK-3)
Vial size: 100 Tests
Concentration: 5 µL/Test
Storage: 4° C, store in dark, DO NOT FREEZE!

CD4 protein structure - 58-0047-42 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Supplement Figure 2. Representative examples of flow cytometric dot plots of surface-stained cells presenting CD4+CCR7+, CD8+CCR7+, CD4+CD62L-, and CD8+CD62L- from the peripheral blood of one patient with adult-onset Still's disease (AOSD), a patient with rheumatoid arthritis (RA), and a healthy control (HC).
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 9 Effect of downmodulation of CD4 and CIB proteins in CD4+ T-lymphocytes on the expression of proteins implicated in HIV-1 entry. Activated CD4+ T-lymphocytes were transduced with the empty vector (pLKO.1) or vectors leading to the expression of the indicated shRNAs, and the surface expression of the following molecules was measured by flow cytometry: CD3, CD4, CXCR4, LFA-1 and alpha4beta7. Intracellular talin expression was also evaluated in permeabilized cells. In each case, the mean fluorescence intensity (MFI) and a representative fluorescence histogram are shown. For MFI, the values represent the mean +- SEM of 4 independent experiments using two different shRNAs per targeted gene and are expressed relative to values obtained for non-transduced cells. *P < 0.05, **P < 0.01 versus sh-SCRAM (Kruskal-Wallis test).
Conjugate: Yellow dye