Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 83-0047-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD4 Monoclonal Antibody (SK3 (SK-3)), eVolve 605, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes. Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis. Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip. Laser/Filter Recommendation: eVolve 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection. Fixation Recommendation: Antibodies conjugated to eVolve are compatible with eBioscience's fixation and permeabilization buffers. Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet Laser Storage and handling: For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SK3 (SK-3)
- Vial size
- 25 Tests
- Concentration
- 5 µl/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0036-42) and Anti-Human CD4 eVolve™ 605. Cells in the lymphocyte gate were used for analysis.