MA1-39582

antibody from Invitrogen Antibodies

Targeting: CD4

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-39582

Provider

Invitrogen Antibodies

Product name

CD4 Monoclonal Antibody (SP35)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil or T Cell lymphoma.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

SP35

Vial size

1 mL

Concentration

0.025 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Combined cART including Tenofovir Disoproxil, Emtricitabine, and Dolutegravir has potent therapeutic effects in HIV-1 infected humanized mice.

Weichseldorfer M, Affram Y, Heredia A, Rikhtegaran-Tehrani Z, Sajadi MM, Williams SP, Tagaya Y, Benedetti F, Ramadhani HO, Denaro F, Munawwar A, Bryant J, Zella D, Reitz M, Romerio F, Latinovic OS
Journal of translational medicine 2021 Oct 30;19(1):453

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Stem cell-derived CAR T cells traffic to HIV reservoirs in macaques.

Barber-Axthelm IM, Barber-Axthelm V, Sze KY, Zhen A, Suryawanshi GW, Chen IS, Zack JA, Kitchen SG, Kiem HP, Peterson CW
JCI insight 2021 Jan 11;6(1)

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Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL-36γ and IL-37.

Lossius AH, Berents TL, Saetre F, Nilsen HR, Bradley M, Asad S, Haraldsen G, Sundnes O, Holm JØ
Experimental dermatology 2021 Feb;30(2):249-261

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CD4 using a monoclonal antibody (Product # MA1-39582).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CD4 using anti-CD4 Monoclonal Antibody (Product # MA5-16338) in Tonsil Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:50.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Multilineage engraftment of HSPC-derived CAR + cells in lymphoid germinal centers. ( A ) Percentages of total B cell (CD20 + ), T cell (CD3 + ), CTL (CD3 + CD8 + ), and monocyte/macrophage (CD68/CD163 + ) immunophenotypic area that colocalized with CD4CAR immunoreactivity in GCs ( n = 4 macaques; 6-7 lymphoid tissues per macaque). The charts show individual data points with medians. ( B ) Representative fluorescent mIHC photomicrographs of CD4CAR (green), CD3 (yellow), CD8 (red), and DAPI nuclear counterstain (blue), from CAR 1 mesenteric LN. Arrowheads indicate colocalization between CD4CAR and CD3/CD8 markers, showing the presence of CD4CAR + CTLs within the germinal center. Scale bars: 50 mum.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Confocal images of p24 in CD45+ lymphocytes or CD4+ T cells from hu-mouse tissues. Shown are a donor PBMC infected in vitro ( A ) and HIV(+) CD45+ ( B ), HIV(-) CD45+ ( C ), HIV(+) CD4+ ( D ), or HIV(-) CD4+ ( E ) lymphocytes or T cells in mouse liver, lymph node, and spleen from infected or uninfected hu-mice. Representative tissue sections were analysed by alpha-p24 mAb in red and human alpha-CD45 or -CD4 in green among the indicated groups of mice. White arrows indicate cells expressing p24 ( A , B , and D ). The bar size is 5 um for PBMCs in panel A and 10 um for tissue sample cells in panels B - E