MA5-15775

antibody from Invitrogen Antibodies

Targeting: CD4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15775

Provider

Invitrogen Antibodies

Product name

CD4 Monoclonal Antibody (8G1B12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control is rat lung tissue lysate.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

8G1B12

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat lung tissue lysate using a CD4/Ly-4 monoclonal antibody (Product # MA5-15775) at (A) 0.5 and (B) 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human, Mouse and Rat Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: CD4 Monoclonal Antibody (8G1B12) (Product # MA5-15775) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human, Mouse and Rat Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: CD4 Monoclonal Antibody (8G1B12) (Product # MA5-15775) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Rat Lung Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: CD4 Monoclonal Antibody (8G1B12) (Product # MA5-15775) (A: 0.5 µg/mL and B: 1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed human thymus tissue labeling CD4 with CD4 Monoclonal Antibody (8G1B12) (Product # MA5-15775) at 5 µg/mL, followed by goat anti-mouse IgG secondary antibody at 1:500 dilution (green), Phylloidin staining (red) and DAPI staining (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human thymus tissue using CD4 Monoclonal Antibody (8G1B12) (Product # MA5-15775) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.