PA5-81490

antibody from Invitrogen Antibodies

Targeting: ARIH2 TRIAD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-81490

Provider

Invitrogen Antibodies

Product name

ARIH2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human ARIH2.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ARIH2 in Lane A: Jurkat Whole Cell Lysate (30 µg), Lane B: K562 Whole Cell Lysate (30 µg), Lane C: Hela Whole Cell Lysate (30 µg). Samples were probed using a ARIH2 Polyclonal Antibody (Product # PA5-81490) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:57 kDa. Observed band size:57 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ARIH2 Polyclonal Antibody (Product # PA5-81490) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of ARIH2 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ARIH2 Polyclonal Antibody (Product # PA5-81490, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ARIH2 in human trachea with ARIH2 Polyclonal Antibody (Product # PA5-81490, 1:500, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ARIH2 in human trachea with ARIH2 Polyclonal Antibody (Product # PA5-81490, 1:500, formalin-fixed paraffin embedded sections).