Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00054606-M05 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00054606-M05, RRID:AB_538220
- Product name
- Mouse Monoclonal DDX56 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DDX56 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 56
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Oncogenic splicing abnormalities induced by DEAD-Box Helicase 56 amplification in colorectal cancer.
Kouyama Y, Masuda T, Fujii A, Ogawa Y, Sato K, Tobo T, Wakiyama H, Yoshikawa Y, Noda M, Tsuruda Y, Kuroda Y, Eguchi H, Ishida F, Kudo SE, Mimori K
Cancer science 2019 Oct;110(10):3132-3144
Cancer science 2019 Oct;110(10):3132-3144
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody (M05), clone 4C5. Lane 1: DDX56 transfected lysatE (62 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in PC-12 (Cat # L012V1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: DDX56 Antibody (4C5) [H00054606-M05] - Detection limit for recombinant GST tagged DDX56 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of monoclonal antibody to DDX56 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml
