PA5-54637

antibody from Invitrogen Antibodies

Targeting: LLGL1 DLG4, HUGL, HUGL-1, Lgl1, LLGL, Mgl1

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-54637 - Provider product page
Provider: Invitrogen Antibodies
Product name: LLGL1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: FERFSLSARN ITEPLCSLDI NWPRDATQAS YRIRESPKLS QANGTPSILL APQSLDGSPD PAHSMGPDTP EPPEAALSPM SIDSATSADT TLDTTGDVTV EDVKDFLGSS EESEKNLRNL AEDE Highest antigen sequence identity to the following orthologs: Mouse - 76%, Rat - 72%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.4 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

LLGL1 protein structure - PA5-54637 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 10. Polarity markers in DLD20-2 cells expressing different sLUR mutants. (a) Representative z-sections of DLD20-2 cells expressing sLUR-517GFP, sLUR-469GFP, sLUR-402GFP, and sLUR-Delta(403-420). The cells were imaged for GFP fluorescence (green) in conjunction with immunostaining for Dlg1, Par6B, and Pals1 (all red). Bars, 10 um. Note that neither of the sLUR-517GFP mutants rescues ABP of DLD20-2 cells. (b) The cells were imaged for GFP (green) and for Llgl1/2 (red). x-y projections of three optical z slices spanning only 1.2 um of the apical cell regions are shown. The entire optical cross-sections of these cells along the white lines are at the bottom. Bars, 10 um. Dashed boxes show regions of higher magnifications (right). Note that some GFP and Llgl1/2 clusters are colocalized (arrowheads). (c) PCC values between green (GFP) and red (Llgl1/2) fluorescence of the random optical z slices of the images shown in b. (d) Western blot of the anti-GFP precipitates obtained from the confluent cultures of the control DLD cells and DLD20-2 cells expressing sLUR-517GFP mutants (marked as in Fig. 8, c and d ) probed for GFP and Llgl1/2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. Both PDZ domain-deficient GFP-sLUR and GFP-Lano rescue defects in polarity protein localization. (a and b) Projections of all optical slices of DLD20-2 cells expressing GFP-sLUR (a) and GFP-Lano (b). Cells were imaged for GFP fluorescence (green) in conjunction with anti-Llgl1/2, Pals1, or Par6B staining (all red). The corresponding x-z sections (with the same magnification) through the middle of each image are shown at the bottom. Bars, 10 um.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 9. Dominant-negative effect of the LAPSDb-deficient mutant. (a) Representative widefield images of DLD1, MDCK, and HBE cells expressing different sLUR mutants (indicated on the left) imaged for GFP (green) and CNG (red). The cells were cultured 48 h before staining. Note that the cells expressing the LAPSDb-deficient mutant, sLUR-469GFP, but not other mutants exhibit dramatic disintegration of TJs. Bars, 30 um. (b) Representative optical z-sections of MDCK cells expressing the full-length sLUR (sLUR-517GFP), and its mutants sLUR-469 GFP and sLUR-402GFP stained for Llgl1/2 (red). Note apical localization of Llgl1/2 in cells expressing sLUR-469GFP. Bars, 10 um. The boxed regions are zoomed on the right panel. Bars, 3 um. Note partial colocalization of the mutant and Llgl1/2 (arrowhead). (c) WT HBE cells stained for hScrib (Scrib) and Llgl1. Note the absence of both at the apical cortex. (d) HBE cells expressing sLUR-469GFP imaged for GFP and Llgl1. Note apical enrichment of both proteins. Bar, 10 um. The boxed area is zoomed on the right. Bar, 5 um.