H00005478-M01

antibody from Abnova Corporation

Targeting: PPIA CYPA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005478-M01

Provider

Abnova Corporation

Proper citation

Product name

PPIA monoclonal antibody (M01), clone 1F4-1B5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PPIA.

Antigen sequence

MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAEN
FRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRH
NGTGGKSIYGEKFEDENFILKHTGPGILSMANAGP
NTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVE
AMERFGSRNGKTSKKITIADCGQLE


Isotype

IgG

Antibody clone number

1F4-1B5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Establishment of a novel cell line for the enhanced production of recombinant adeno-associated virus vectors for gene therapy.

Satkunanathan S, Wheeler J, Thorpe R, Zhao Y
Human gene therapy 2014 Nov;25(11):929-41

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Peripheral blood monocyte-expressed ANXA2 gene is involved in pathogenesis of osteoporosis in humans.

Deng FY, Lei SF, Zhang Y, Zhang YL, Zheng YP, Zhang LS, Pan R, Wang L, Tian Q, Shen H, Zhao M, Lundberg YW, Liu YZ, Papasian CJ, Deng HW
Molecular & cellular proteomics : MCP 2011 Nov;10(11):M111.011700

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Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma.

Rho JH, Roehrl MH, Wang JY
Journal of proteome research 2009 Dec;8(12):5610-8

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PPIA monoclonal antibody (M01), clone 1F4-1B5 Western Blot analysis of PPIA expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PPIA expression in transfected 293T cell line by PPIA monoclonal antibody (M01), clone 1F4-1B5.Lane 1: PPIA transfected lysate(18 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PPIA is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol