AP1477a

antibody from Abcepta

Targeting: CDH1 CD324, UVO, uvomorulin

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1477a

Provider

Abcepta

Proper citation

Abgent Cat#AP1477a, RRID:AB_2244626

Product name

E Cadherin (CDH1) Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of CDH1 Antibody (N-term) (Cat.#AP1477a) in 293 cell line lysates (35ug/lane). CDH1 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of CDH1 (arrow) using rabbit polyclonal CDH1 Antibody (N-term) (Cat.#AP1477a).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50