PB9561

antibody from Boster Biological Technology

Targeting: CDH1 CD324, UVO, uvomorulin

Provider product page for PB9561

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [49]
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Immunohistochemistry [1]

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Product number: PB9561 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-E Cadherin 1/CDH1 Antibody Picoband™
Antibody type: Polyclonal
Description: Polyclonal antibody for E CADHERIN/CDH1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, IF, ELISA. Reactive species: Human;Mouse;Rat. E CADHERIN/CDH1 information: Molecular Weight: 97456 MW; Subcellular Localization: Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane; Tissue Specificity: Non-neural epithelial tissues.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Vial size: 100μg/vial
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

CDH1 protein structure - PB9561 shown in red.

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Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: IHC analysis of E Cadherin using anti-E Cadherin antibody (PB9561). E Cadherin was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Additional image