14-3249-80

antibody from Invitrogen Antibodies
Targeting: CDH1 CD324, UVO, uvomorulin
Provider product page for 14-3249-80
Western blot
ELISA
Immunocytochemistry
Immunoprecipitation
Immunohistochemistry
Flow cytometry
Other assay

Antibody data

Product number
14-3249-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton. Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers. E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided. The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells. Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, western blotting, immunohistochemical staining of frozen tissue sections, and ELISA. (Please use Functional Grade purified DECMA-1, Product # 16-3249-85 , in functional assays). Applications Tested: This DECMA-1 antibody has been tested by western blot analysis of EDTA treated MDCK cell line. This can be used at less than or equal to 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse, Canine
Host
Rat
Isotype
IgG
Antibody clone number
DECMA-1
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
CDH1 protein structure - 14-3249-80 shown in red.