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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00005886-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005886-M01, RRID:AB_539662
- Product name
- Mouse Monoclonal Rad23 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RAD23A - RAD23 homolog A (S. cerevisiae)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Human Rad23A plays a regulatory role in autophagy.
hHR23A is required to control the basal turnover of Chk1.
Cisplatin transiently up-regulates hHR23 expression through enhanced translational efficiency in A549 adenocarcinoma cells.
Tan X, Wang HC, Liang RY, Chuang SM
Biochemical and biophysical research communications 2016 Sep 30;478(4):1772-9
Biochemical and biophysical research communications 2016 Sep 30;478(4):1772-9
hHR23A is required to control the basal turnover of Chk1.
Tan X, Liang RY, Chuang SM
Cellular signalling 2015 Nov;27(11):2304-13
Cellular signalling 2015 Nov;27(11):2304-13
Cisplatin transiently up-regulates hHR23 expression through enhanced translational efficiency in A549 adenocarcinoma cells.
Shen YH, Chen BR, Cherng SH, Chueh PJ, Tan X, Lin YW, Lin JC, Chuang SM
Toxicology letters 2011 Sep 10;205(3):341-50
Toxicology letters 2011 Sep 10;205(3):341-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHR23A Antibody (3C12) [H00005886-M01] - Analysis of RAD23A expression in transfected 293T cell line by RAD23A monoclonal antibody (M01), clone 3C12. Lane 1: RAD23A transfected lysate (39.6 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHR23A Antibody (3C12) [H00005886-M01] - Analysis of RAD23A over-expressed 293 cell line, cotransfected with RAD23A Validated Chimera RNAi ( Cat # H00005886-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD23A monoclonal antibody (M01), clone 3C12 (Cat # H00005886-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: hHR23A Antibody (3C12) [H00005886-M01] - Detection limit for recombinant GST tagged RAD23A is approximately 0.03ng/ml as a capture antibody.
