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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [3]
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- Product number
- RQ6895 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- hHR23A Antibody / RAD23A
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the hHR23A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human K562, 2) human HeLa, 3) human MCF7, 4) human Jurkat, 5) rat testis, 6) mouse testis and 7) mouse lung tissue lysate with hHR23A antibody. Predicted molecular weight ~40 kDa but commonly observed at 40-55 kDa and possibly larger due to ubiquitination.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with hHR23A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= hHR23A antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse ANA-1 cells with hHR23A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= hHR23A antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of rat C6 cells with hHR23A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= hHR23A antibody.
