Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-77744 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EDG3 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references Upregulation of sphingosine-1-phosphate receptor 3 on fibroblast-like synoviocytes is associated with the development of collagen-induced arthritis via increased interleukin-6 production.
Inoue T, Kohno M, Nagahara H, Murakami K, Sagawa T, Kasahara A, Kaneshita S, Kida T, Fujioka K, Wada M, Nakada H, Hla T, Kawahito Y
PloS one 2019;14(6):e0218090
PloS one 2019;14(6):e0218090
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat heart (lanes 1 and 3), rat kidney (lanes 2 and 4), Jurkat (lanes 5 and 7) and WEHI (lanes 6 and 8) lysates with EDG3 (extracellular) polyclonal antibody (Product # PA5-77744) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Raji cells using polyclonal EDG3 (extracellular) antibody (Product # PA5-77744) with a dilution of 5 µg/5x10^5cells.