LS-C133038

antibody from LSBio

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C133038

Provider

LSBio

Product name

AKT1 Antibody (clone 2E11) LS-C133038

Antibody type

Monoclonal

Description

Purified from ascites by Protein A

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2E11

Storage

Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of AKT1 expression in HeLa cell lysates.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of AKT1 expression in HepG2 cell lysates.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of AKT1 expression in transfected 293T cell line by AKT1 monoclonal antibody, clone 2E11.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AKT1 is approximately 3 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AKT1 is approximately 3 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AKT1 is approximately 3 ng/ml as a capture antibody.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation of AKT1 transfected lysate using anti-AKT1 monoclonal antibody and Protein A Magnetic Bead.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of AKT1 transfected lysate using anti-AKT1 monoclonal antibody and Protein A Magnetic Bead.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of AKT1 transfected lysate using anti-AKT1 monoclonal antibody and Protein A Magnetic Bead.