Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-21678 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal AKT1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [Biotin] [NBP2-21678] - GST tagged AKT1 recombinant protein, 25 ng. AKT1 phospho S473 Biotin Conjugated antibody at 1:1000, overnight at 4C. Streptavidin-HRP secondary antibody at 1:40,000 for 30 min at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [Biotin] [NBP2-21678] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [Biotin] [NBP2-21678] - Analysis of FFPE prostate tissue (left panel). Antibody at 20 ug/mL. Antigen retrieval in citrate buffer pH 6.2 and heat/pressure. Streptavidin-HRP secondary antibody at 10 ug/mL. Hematoxylin nuclear counterstain. Left panel: negative control.
