Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX110613 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110613, RRID:AB_10617128
- Product name
- AKT1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Copanlisib, a novel phosphoinositide 3-kinase inhibitor, combined with carfilzomib inhibits multiple myeloma cell proliferation.
MicroRNA-296 inhibits colorectal cancer cell growth and enhances apoptosis by targeting ARRB1-mediated AKT activation.
A high-throughput pipeline for validation of antibodies.
Erythropoietin protects neuroblastoma cells against etoposide and vincristine by activating ERK and AKT pathways but has no effect in kidney cells.
A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
The synthetic flavonoid WYC02-9 inhibits cervical cancer cell migration/invasion and angiogenesis via MAPK14 signaling.
Okabe S, Tanaka Y, Tauchi T, Ohyashiki K
Annals of hematology 2019 Mar;98(3):723-733
Annals of hematology 2019 Mar;98(3):723-733
MicroRNA-296 inhibits colorectal cancer cell growth and enhances apoptosis by targeting ARRB1-mediated AKT activation.
Zhang Z, Zhong X, Xiao Y, Chen C
Oncology reports 2019 Jan;41(1):619-629
Oncology reports 2019 Jan;41(1):619-629
A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Erythropoietin protects neuroblastoma cells against etoposide and vincristine by activating ERK and AKT pathways but has no effect in kidney cells.
Vazquez-Mellado MJ, Aguilar C, Rocha-Zavaleta L
Life sciences 2015 Sep 15;137:142-9
Life sciences 2015 Sep 15;137:142-9
A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Lee KH, Lo HL, Tang WC, Hsiao HH, Yang PM
Scientific reports 2014 Sep 17;4:6394
Scientific reports 2014 Sep 17;4:6394
The synthetic flavonoid WYC02-9 inhibits cervical cancer cell migration/invasion and angiogenesis via MAPK14 signaling.
Chen YJ, Cheng YJ, Hung AC, Wu YC, Hou MF, Tyan YC, Yuan SS
Gynecologic oncology 2013 Dec;131(3):734-43
Gynecologic oncology 2013 Dec;131(3):734-43
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (20 ug of whole cell lysate) A: HeLa cell lysate B: HeLa cell transfect with AKT 7.5% SDS PAGE GTX110613 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX110613 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Akt1 antibody detects Akt1 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Akt1 protein stained by Akt1 antibody (GTX110613) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Akt1 antibody immunoprecipitates Akt1 protein in IP experiments.IP samples: 30 £gg whole cell extract of Akt1-transfected 293T cells.A. 30 £gg whole cell extract of Akt1-protein expressing 293T cellB. Control with 3 £gg of preimmune Rabbit IgGC. Immunoprecipitation of Akt1 protein by 3 £gg Akt1 antibody (GTX110613)10 % SDS-PAGEThe immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Akt1(GTX110613) antibody at 1:250 dilution.