Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 44-602G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-AKT1 (Thr308) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Selective Phosphorylation of Akt/Protein-Kinase B Isoforms in Response to Dietary Cues.
2,5-Dimethyl-Celecoxib Extends Drosophila Life Span via a Mechanism That Requires Insulin and Target of Rapamycin Signaling.
Possible Mechanisms of Di(2-ethylhexyl) Phthalate-Induced MMP-2 and MMP-9 Expression in A7r5 Rat Vascular Smooth Muscle Cells.
Insulin signaling in type 2 diabetes: experimental and modeling analyses reveal mechanisms of insulin resistance in human adipocytes.
Constitutive K-RasG12D activation of ERK2 specifically regulates 3D invasion of human pancreatic cancer cells via MMP-1.
Reconstitution of PTEN activity by CK2 inhibitors and interference with the PI3-K/Akt cascade counteract the antiapoptotic effect of human stromal cells in chronic lymphocytic leukemia.
Phosphoinositide3-kinase regulates actin polymerization during delayed phagocytosis of Helicobacter pylori.
Trautenberg LC, Prince E, Maas C, Beier N, Honold F, Grzybek M, Brankatschk M
Frontiers in cell and developmental biology 2019;7:206
Frontiers in cell and developmental biology 2019;7:206
2,5-Dimethyl-Celecoxib Extends Drosophila Life Span via a Mechanism That Requires Insulin and Target of Rapamycin Signaling.
Wu Q, Lian T, Fan X, Song C, Gaur U, Mao X, Yang D, Piper MDW, Yang M
The journals of gerontology. Series A, Biological sciences and medical sciences 2017 Oct 1;72(10):1334-1341
The journals of gerontology. Series A, Biological sciences and medical sciences 2017 Oct 1;72(10):1334-1341
Possible Mechanisms of Di(2-ethylhexyl) Phthalate-Induced MMP-2 and MMP-9 Expression in A7r5 Rat Vascular Smooth Muscle Cells.
Shih MF, Pan KH, Cherng JY
International journal of molecular sciences 2015 Dec 4;16(12):28800-11
International journal of molecular sciences 2015 Dec 4;16(12):28800-11
Insulin signaling in type 2 diabetes: experimental and modeling analyses reveal mechanisms of insulin resistance in human adipocytes.
BrÀnnmark C, Nyman E, Fagerholm S, Bergenholm L, Ekstrand EM, Cedersund G, StrÄlfors P
The Journal of biological chemistry 2013 Apr 5;288(14):9867-9880
The Journal of biological chemistry 2013 Apr 5;288(14):9867-9880
Constitutive K-RasG12D activation of ERK2 specifically regulates 3D invasion of human pancreatic cancer cells via MMP-1.
Botta GP, Reginato MJ, Reichert M, Rustgi AK, Lelkes PI
Molecular cancer research : MCR 2012 Feb;10(2):183-96
Molecular cancer research : MCR 2012 Feb;10(2):183-96
Reconstitution of PTEN activity by CK2 inhibitors and interference with the PI3-K/Akt cascade counteract the antiapoptotic effect of human stromal cells in chronic lymphocytic leukemia.
Shehata M, Schnabl S, Demirtas D, Hilgarth M, Hubmann R, Ponath E, Badrnya S, Lehner C, Hoelbl A, Duechler M, Gaiger A, Zielinski C, Schwarzmeier JD, Jaeger U
Blood 2010 Oct 7;116(14):2513-21
Blood 2010 Oct 7;116(14):2513-21
Phosphoinositide3-kinase regulates actin polymerization during delayed phagocytosis of Helicobacter pylori.
Allen LA, Allgood JA, Han X, Wittine LM
Journal of leukocyte biology 2005 Jul;78(1):220-30
Journal of leukocyte biology 2005 Jul;78(1):220-30
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition and Phosphatase Stripping: Extracts prepared from HEK293 cells co-expressing RasV12 and RacL61 were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were left untreated (1-5) or treated with lambda (&
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-AKT1 (Thr308) using a polyclonal antibody (Product # 44-602G).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of AKT/PKB (pT308) showing staining in the cytoplasm of paraffin-embedded mouse prostate tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a AKT/PKB (pT308) Rabbit Polyclonal Antibody (Product # 44-602G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of AKT/PKB (pT308) showing staining in the cytoplasm and nucleus of paraffin-embedded human prostate carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a AKT/PKB (pT308) Rabbit Polyclonal Antibody (Product # 44-602G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of AKT/PKB (pT308) showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a AKT/PKB (pT308) Rabbit Polyclonal Antibody (Product # 44-602G) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Treat cells with desired stimulant. The concentration and time of stimulation will vary depending on desired effect.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Effects of DEHP on phosphorylated p38 MAPK, phosphorylated ERK1/2, phosphorylated Akt, and NF-kappaB (p65) protein expression. VSMC ( n >= 3) were treated with DEHP (concentrations between 2 and 17.5 ppm) for 20 min (p38 MAPK, ERK1/2, and Akt) or 12 h (NF-kappaB) prior to protein extraction. Phosphorylated p38 MAPK, phosphorylated ERK1/2, phosphorylated Akt, and NF-kappaB (p65) were expressed by Western blotting. Statistics are shown for DEHP-treated cells * p < 0.05, ** p < 0.01, and *** p < 0.005, compared to the respective control groups.