44-623G

Zhao Z, Ma J, Mao Y, Dong L, Li S, Zhang Y
Medical science monitor : international medical journal of experimental and clinical research 2018 Sep 27;24:6851-6860

Regulation of H-Ras-driven MAPK signaling, transformation and tumorigenesis, but not PI3K signaling and tumor progression, by plasma membrane microdomains.

Michael JV, Wurtzel JG, Goldfinger LE
Oncogenesis 2016 May 30;5(5):e228

Differential mTOR and ERK pathway utilization by effector CD4 T cells suggests combinatorial drug therapy of arthritis.

Lin JT, Stein EA, Wong MT, Kalpathy KJ, Su LL, Utz PJ, Robinson WH, Fathman CG
Clinical immunology (Orlando, Fla.) 2012 Feb;142(2):127-38

Naive CD4 t cell proliferation is controlled by mammalian target of rapamycin regulation of GRAIL expression.

Lin JT, Lineberry NB, Kattah MG, Su LL, Utz PJ, Fathman CG, Wu L
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):5919-28

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts of NIH3T3 cells using rabbit anti-Akt [pS473] antibody (Product # 44-623G). NIH3T3 cells untreated (lane 1) or treated with PDGF (50 ng/mL, 15 min) (lanes 2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Prior to incubation with the primary antibody, the membrane was incubated with no peptide (lane 1 and 2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), a generic phosphoserine-containing peptide (lane 4), or the phosphopeptide immunogen (lane 5).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts of NIH3T3 cells using rabbit anti-Akt [pS473] antibody (Product # 44-623G). NIH3T3 cells untreated (lane 1) or treated with PDGF (50 ng/mL, 15 min) (lanes 2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Prior to incubation with the primary antibody, the membrane was incubated with no peptide (lane 1 and 2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), a generic phosphoserine-containing peptide (lane 4), or the phosphopeptide immunogen (lane 5).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Akt/PKB [pS473] was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Tritonª X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Akt/PKB [pS473] Rabbit Polyclonal Antibody (44623G, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor¨ 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune¨ Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL