AM00110PU-N

antibody from Acris Antibodies GmbH

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AM00110PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00110PU-N, RRID:AB_972604

Product name

anti AKT1 / PKB (466-480) (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic peptide conjugated to KLH. Epitope: C-terminus (aa 466-480).

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

5C10

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Detection of endogenous PKB Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab PKB-5C10 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). lane 1: SW480; lane 2: SW620; lane 3: HT29; lane 4: MCF-7; lane 5: MDA-MB 231; lane 6: T47D