AM08435PU-N

antibody from Acris Antibodies GmbH

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AM08435PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM08435PU-N, RRID:AB_2035090

Product name

anti AKT1 / PKB

Antibody type

Monoclonal

Antigen

Synthetic peptide corresponding to residues near the C terminal end of of Human AKT1 protein

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

18F10.E5

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Anti-AKT monoclonal antibody shows detection of AKT (indicated by arrowhead at ~56 kDa) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 µg of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO in TBS for 2h at RT. The membrane was probed with the primary antibody at a 1:1,000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax(TM) enhanced chemiluminescent reagent. Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.)