AP09425PU-N

antibody from Acris Antibodies GmbH

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09425PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09425PU-N, RRID:AB_2035092

Product name

anti AKT1 / PKB

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Sheep

Isotype

IgG

Vial size

0.1 mg

Concentration

0.9 mg/ml (by UV absorbance at 280 nm)

References

Submitted references

Mechanosensitive kinases regulate stiffness-induced cardiomyocyte maturation.

Young JL, Kretchmer K, Ondeck MG, Zambon AC, Engler AJ
Scientific reports 2014 Sep 19;4:6425

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using affinity purified anti-AKT antibody shows detection of endogenous protein in whole cell extracts from H2O2 treated MCF7 cells (lane 2). Recombinant AKT is detected in lane 3. Lanes 1 and 4 contain wcl from HeLa and A431 cells, respectively. Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown). The band at ~55-60 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT. Primary antibody was diluted 1:300 in 1% BLOTTO and reacted with the membrane overnight at 4° C. IRDYE800(TM) conjugated Dnky-a-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature.