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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM05348PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM05348PU-N, RRID:AB_1609576
- Product name
- anti AKT1 / PKB
- Antibody type
- Monoclonal
- Antigen
- Recombinant human AKT1 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9Q7
- Vial size
- 0.1 mg
- Concentration
- Lot specific
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Proteins from cell extracts of human Jurkat cells (lane 1), mouse 3T3-L1 cells (lane 2), and rat PC-12 cells (lane 3) were resolved by SDS-PAGE and transferred to PVDF. The membranes were incubated with this AKT monoclonal antibody (clone 9Q7) at a concentration of 1 μg/mL for two hours at room temperature. After washing, the membranes were incubated with a goat F(abâ)2 anti-mouse IgG alkaline phosphatase conjugated antibody at a 1:2000 dilution. Bands were detected with CDP-substrate using the WesternStarTM method (Tropix)
