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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP50136PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP50136PU-N, RRID:AB_11146054
- Product name
- anti AKT1 / PKB
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 289-318 amino acids from human AKT1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AKT1 (Thr308) Antibody (Cat. #AP50136PU-N) western blot analysis in MDA-MB435 cell line lysates (35µg/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AKT1 antibody (Thr308) (Cat. #AP50136PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the AKT1 antibody (Thr308) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AKT1 Antibody (Thr308) (Cat. #AP50136PU-N) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
