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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP50137PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP50137PU-N, RRID:AB_11147190
- Product name
- anti AKT1 / PKB (C-term)/(C-term T450)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the C-terminal region of human AKT1
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AKT1 Antibody (pT450) (Cat. #AP50137PU-N) western blot analysis in MCF-7 cell line lysates (35 µg/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of AKT1 Antibody (C-term T450)(Cat#AP50137PU-N) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AKT1 Antibody (C-term T450) (Cat. #AP50137PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKT1 Antibody (C-term T450) for immunohistochemistry. Clinical relevance has not been evaluated.
