GTX48651

antibody from GeneTex

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

GTX48651

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48651, RRID:AB_11175629

Product name

AKT antibody

Antibody type

Polyclonal

Antigen

Other

Host

Sheep

Isotype

IgG

Vial size

50µg

Storage

Store vial at 4° C for short-term use.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Anti-AKT Antibody in western blot showing detection of endogenous protein in whole cell extracts from H2O2 treated MCF7 cells (lane 2). Recombinant AKT is detected in lane 3. Lanes 1 and 4 contain wcl from HeLa and A431 cells, respectively. Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown). The band at ~55-60 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT. AKT antibody was diluted 1:300 in 1% BLOTTO and reacted with the membrane overnight at 4° C. IRDYE800 conjugated Dnky-anti-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature.

Validation comment

WB