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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F50139 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- AKT1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 115-144 from the human protein was used as the immunogen for this AKT1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Store at 4°C for up to one month. For long term, aliquot the AKT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of AKT1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF testing of AKT1 antibody with MDA-MB435 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments stained red; DAPI nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human breast carcinoma stained with AKT1 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- AKT1 antibody flow cytometric analysis of MDA-MB435 cells (green) compared to a negative control (blue).
