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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- ARG10548 - Provider product page
- Provider
- Arigo
- Product name
- anti-Akt1 antibody [C20-A]
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide around the C-terminus of Human AKT1 protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- C20-A
- Vial size
- 50 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 1) 100 ng of recombinant Human Akt1 (His-tagged), 2) 20 µg, 3) 100 µg, and 4) 200 µg of Mouse brain extracts stained with ARG10548 anti-Akt1 antibody [C20-A].
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: HEK293 cells stained with ARG10548 anti-Akt1 antibody [C20-A] at 1:300 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: 1) 50 ng of recombinant Akt1, 2) 200 µg of Mouse brain extract, immuprecipitation protocol followed without the primary antibody (negative control). 3) Akt1 kinase immunoprecipitation from 200 µg of Mouse brain protein extract with ARG10548 anti-Akt1 antibody [C20-A].* Dobransky T et al (2003) Phosphorylation of 69 kDa choline acetyltransferase at threonine-456 in response to short-term exposure to amyloid-? peptide 1-42. J Biol Chem 278, 5883-5893.
