PAB4548

antibody from Abnova Corporation

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB4548

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB4548, RRID:AB_1571061

Product name

AKT1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of AKT1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Akt activation disrupts mammary acinar architecture and enhances proliferation in an mTOR-dependent manner.

Debnath J, Walker SJ, Brugge JS
The Journal of cell biology 2003 Oct 27;163(2):315-26

---

Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.

Powell DJ, Hajduch E, Kular G, Hundal HS
Molecular and cellular biology 2003 Nov;23(21):7794-808

---

Increase of AKT/PKB expression correlates with gleason pattern in human prostate cancer.

Liao Y, Grobholz R, Abel U, Trojan L, Michel MS, Angel P, Mayer D
International journal of cancer 2003 Nov 20;107(4):676-80

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of AKT1 (arrow) using AKT1 polyclonal antibody (Cat # PAB4548).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Y-79 cell lysate (35 ug/lane) with AKT1 polyclonal antibody (Cat # PAB4548).