PAB4918

antibody from Abnova Corporation

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB4918

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB4918, RRID:AB_1577834

Product name

AKT1 (phospho S473) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Akt activation disrupts mammary acinar architecture and enhances proliferation in an mTOR-dependent manner.

Debnath J, Walker SJ, Brugge JS
The Journal of cell biology 2003 Oct 27;163(2):315-26

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Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.

Powell DJ, Hajduch E, Kular G, Hundal HS
Molecular and cellular biology 2003 Nov;23(21):7794-808

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Increase of AKT/PKB expression correlates with gleason pattern in human prostate cancer.

Liao Y, Grobholz R, Abel U, Trojan L, Michel MS, Angel P, Mayer D
International journal of cancer 2003 Nov 20;107(4):676-80

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of AKT1 (phospho S473) polyclonal antibody (Cat # PAB4918) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)