MAB1919

antibody from Abnova Corporation

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1919

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB1919, RRID:AB_1678449

Product name

AKT1 (phospho S473) monoclonal antibody, clone 17B6.B11.A12.C3.G7.G12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against synthetic phosphopeptide of AKT1.

Isotype

IgG

Antibody clone number

17B6.B11.A12.C3.G7.G12

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture.

Alessi DR
Biochemical Society transactions 2001 May;29(Pt 2):1-14

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PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?

Lawlor MA, Alessi DR
Journal of cell science 2001 Aug;114(Pt 16):2903-10

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Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.

Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA
Proceedings of the National Academy of Sciences of the United States of America 1991 May 15;88(10):4171-5

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using AKT1 (phospho S473) monoclonal antibody, clone 17B6.B11.A12.C3.G7.G12 (Cat # MAB1919) shows detection of phospho-AKT1 (indicated by arrowhead at ~56 KDa) on PDGF stimulated NIH/3T3 cell lysates (Lane 2).No reactivity is seen for non-phosphorylated AKT1 in untreated cells (Lane 1).Each lane contained approximately 10 ug of lysate.All samples were loaded on to a 4-20% gradient gel for separation.After electrophoresis, the gel was blocked with 5% BLOTTO in TBS for 90 min at RT.The membrane was probed with the primary antibody at a 1:10,000 dilution in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4°C. For detection HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4°C with FemtoMax™ enhanced chemiluminescent reagent.Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry of formalin fixed and paraffin embedded using AKT1 (phospho S473) monoclonal antibody, clone 17B6.B11.A12.C3.G7.G12 (Cat # MAB1919) shows detection of Phospho-AKT1 S473 in human prostate tissue.The antibody was used at 20 ug/mL .The staining is much stronger than the weak basal level of phosphorylation in normal prostate tissue.Signal was developed using Dako's Techmate streptavidin-biotin reagents.Personal communication, Glenna Burmer, Lifespan Biosciences, Seattle, WA.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)