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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB10323 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10323, RRID:AB_1676194
- Product name
- AKT1 (phospho S473) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?
Lawlor MA, Alessi DR
Journal of cell science 2001 Aug;114(Pt 16):2903-10
Journal of cell science 2001 Aug;114(Pt 16):2903-10
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used at a 1:200 dilution to detect phosphorylated AKT1 by Western blot. A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT1 kinase was used.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence microscopy.Confocal image using AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) at a 1 : 40 dilution to detect phosphorylated AKT1 (green) in rat cardiomyocytes infected with adenovirus expressing with wild-type AKT1 in conjunction with a texas-red conjugated phalloidin (red) to label filamentous actin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used at a 1 : 100 dilution to detect AKT1 by immunohistochemistry of formalin-fixed and paraffin embedded in (A) human breast tumor tissue, (B) a higher magnification and (C) normal human breast tissue of the breast tumor.The staining is much stronger than the weak basal level of phosphorylation in normal breast.AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used with no pretreatment of tissue.Signal was developed using Dako's Techmate streptavidin-biotin reagents.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
