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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-61475 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal AKT1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody reacts to AKT1 only when phosphorylated at Ser473. There is no cross-reactivity with AKT1 without phosphorylation at Ser473. This antibody may also react to bovine, mouse or rat phospho-AKT1 (Ser473), as predicted by immunogen homology.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT1 [p Ser473] Antibody (251) [NBP2-61475] - Analysis in lysates from 293 cells either, grown in medium with serum, serum starved, or insulin treated.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT1 [p Ser473] Antibody (RM251) [NBP2-61475] - WB of lysates from 293 cells either grown in medium with serum, serum starved, or insulin treated using AKT1 phospho Ser 473 antibody NBP2-61475.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (RM251) [NBP2-61475] - IHC-P staining of FFPE human breast cancer tissue sections using AKT1 phospho Ser473 antibody NBP2-61475.
