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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- STJ99156 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-AKT1 antibody [2D9-D2-H3] (STJ99156)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Rac-Alpha Serine/Threonine-Protein Kinase is suitable for use in Western Blot research applications.
- Reactivity
- Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of AKT1 in CHO-K1 cell lysate (B) and CHO-K1 transfected by AKT1 (A) cell lysate using AKT1 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of AKT1 in 3T3 cell lysates using AKT1 mouse mAb (dilution 1:2000) , with super ECL.Predicted band size:60KDa.Observed band size:60KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
