ABIN307039

antibody from antibodies-online

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN307039

Provider

antibodies-online

Product name

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

9Q7

Vial size

100 µg

Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Proteins from cell extracts of human Jurkat cells (lane 1), mouse 3T3-L1 cells (lane 2), and rat PC-12 cells (lane 3) were resolved by SDS- PAGE and transferred to PVDF. The membranes were incubated with this AKT monoclonal antibody (clone 9Q7) at a concentration of 1 ?g/mL for two hours at room temperature. After washing, the membranes were incubated with a goat F(ab?)2 anti- mouse IgG alkaline phosphatase conjugated antibody and Kodak BioMax film. others not tested