Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN307039 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9Q7
- Vial size
- 100 µg
- Storage
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Proteins from cell extracts of human Jurkat cells (lane 1), mouse 3T3-L1 cells (lane 2), and rat PC-12 cells (lane 3) were resolved by SDS- PAGE and transferred to PVDF. The membranes were incubated with this AKT monoclonal antibody (clone 9Q7) at a concentration of 1 ?g/mL for two hours at room temperature. After washing, the membranes were incubated with a goat F(ab?)2 anti- mouse IgG alkaline phosphatase conjugated antibody and Kodak BioMax film. others not tested