Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- ABIN307095 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 14-jun
- Vial size
- 10 Miniblots
- Storage
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Submitted references Mechanism of zinc-induced phosphorylation of p70 S6 kinase and glycogen synthase kinase 3beta in SH-SY5Y neuroblastoma cells.
Sef inhibits fibroblast growth factor signaling by inhibiting FGFR1 tyrosine phosphorylation and subsequent ERK activation.
An WL, Bjorkdahl C, Liu R, Cowburn RF, Winblad B, Pei JJ
Journal of neurochemistry 2005 Mar;92(5):1104-15
Journal of neurochemistry 2005 Mar;92(5):1104-15
Sef inhibits fibroblast growth factor signaling by inhibiting FGFR1 tyrosine phosphorylation and subsequent ERK activation.
Kovalenko D, Yang X, Nadeau RJ, Harkins LK, Friesel R
The Journal of biological chemistry 2003 Apr 18;278(16):14087-91
The Journal of biological chemistry 2003 Apr 18;278(16):14087-91
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Immunofluorescence Staining Serum-starved NIH3T3 cells left untreated (left) or treated with PDGF (right) were fixed prior to immunostaining with the Akt [pS473] rabbit monoclonal antibody. The signal was detected with an anti- rabbit FITC conjugated secondary antibody. The data show that the antibody detected phosphorylated Akt in PDGF-treated NIH3T3 cells. Cells were counterstained with DAPI. Please visit our website (biosource.com) to view the images in full color.