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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [3]
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- Product number
- LS-C744941 - Provider product page
- Provider
- LSBio
- Product name
- AKT1 Antibody (Internal, clone 14E5.A2.B2.H9, RPE) LS-C744941
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 14E5.A2.B2.H9
- Storage
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Plate was coated with monoclonal anti AKT1 antibody followed by incubation with recombinant AKT1, AKT2, AKT3 proteins. Binding was detected with biotinylated monoclonal anti AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Plate was coated with monoclonal anti AKT1 antibody followed by incubation with recombinant AKT1, AKT2, AKT3 proteins. Binding was detected with biotinylated monoclonal anti AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Plate was coated with monoclonal anti AKT1 antibody followed by incubation with recombinant AKT1, AKT2, AKT3 proteins. Binding was detected with biotinylated monoclonal anti AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3
